Depletions of TTF2 and FBXO11 sensitize latent provirus in Jurkat 2D10 cell line to re-activation. Duplicate Jurkat 2D10 cultures were transfected with control siRNA or siRNA against TTF2 and FBXO11 for 36 hours. Cells were then activated with suboptimal amounts (2μl) of CD3/CD28 beads and GFP expression from re-activated provirus was analysed by flow cytometry. A representative experiment from two independent experiments is shown. (A) The panel shows GFP expression in untreated cells (background GFP expression) and in activated cells treated with either control siRNA or TTF2 and FBXO11 siRNA at the indicated time points. The x-axis is GFP, and the y-axis is PE that is used as a placeholder fluorophore to enable visualization as a dot plot. A representative flow cytometric analysis of GFP expression from the duplicate samples is shown. (B) The graph represents average GFP expression in cells that were transfected with indicated siRNA. The untreated cells represent background GFP expression. Statistical significance was computed using the t-test (* represent p≤ 0.05 and ** represents p< 0.005).