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Figure 5 | Retrovirology

Figure 5

From: Identification of novel CDK9 and Cyclin T1-associated protein complexes (CCAPs) whose siRNA depletion enhances HIV-1 Tat function

Figure 5

Enhancement of Tat activation by CCAP depletions is not due to increased Tat expression or differential association of CDK9/CCNT1 with 7SK/HEXIM1. (A) HeLa cells were transfected with indicated siRNAs for 48 hours followed by transfection of Flag-tagged wild type HIV-1 Tat expression plasmid. At 24 hours post-plasmid transfection, cell lysates were prepared and immunoblot analysis carried out for expression levels of Flag-Tat, CCNT1, HEXIM1 and PPP1R10; β-actin was probed as loading control. (B) HeLa cells were transfected with control or TTF2+ FBXO11 siRNAs and 48 hours later transfected with expression plasmids for HA-CCNT1 or Flag-wild type Tat (wtTat) or mutant Tat (mTat) for a further 48 hours. Cell lysates were prepared and immunoprecipitated with CDK9. Left part of the panel shows input levels, and right part shows the immunoprecipitated proteins.

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Retrovirology

ISSN: 1742-4690

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