Figure 4

Enhancement of Tat activation by CCAP depletions is not due to activation of NF-κB. HeLa cells were transfected for 48 hours with control siRNA or the indicated siRNAs. Cells were then co-transfected with 500 ng HIV-1 LTR-Luciferase reporter plasmid plus 50 ng wtTat plasmid or 500 ng NF-κB-Luciferase reporter plasmid plus 50 ng pCMV parent vector. Cell extracts were prepared 24 hours post-plasmid transfection, and Luciferase expression was normalized to total cellular protein in cell extracts. Statistical significance was determined using a t-test. A representative experiment from three independent experiments is shown (* represents p value ≤0.05).