The solution structure of the prototype foamy virus RNase H domain indicates an important role of the basic loop in substrate binding

Table 1 Solution structure statistics

Experimentally derived restraints
distance restraints
	NOE	1686
	intraresidual	527
	sequential	343
	medium range	245
	long range	505
	ambiguous	66
	hydrogen bonds	62
dihedral restraints		225
Restraint violation
average distance restraint violation (Å)	0.0035 +/− 0.0008
maximum distance restraint violation (Å)	< 0.1
average dihedral restraint violation (°)	0.10 +/− 0.04
maximum dihedral restraint violation (°)	2.4
deviation from ideal geometry
bond length (Å)	0.00064 +/− 0.00005
bond angle (Å)	0.12 +/− 0.006
Coordinate precision ^a,b
backbone heavy atoms (Rmsd) (Å) (all / defined secondary structure )	1.05 / 0.46
all heavy atoms (Rmsd) (Å)	1.58 / 0.92
Ramachandran plot statistics^c (%)	89.7/9.6/0.3/0.4

^a The precision of the coordinates is defined as the average atomic root mean square difference between the accepted simulated annealing structures and the corresponding mean structure calculated for the given sequence regions.
^b calculated for residues 593–747 (all) or 594–604, 612–621, 629–637, 642–659, 664–668, 671–686, 700–712, 717–720, 731–747 (defined secondary structure).
^c Ramachandran plot statistics are determined by PROCHECK and noted by most favored/additionally allowed/generously allowed/disallowed.

ISSN: 1742-4690