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Figure 5 | Retrovirology

Figure 5

From: The solution structure of the prototype foamy virus RNase H domain indicates an important role of the basic loop in substrate binding

Figure 5

Modeling of an RNA/DNA substrate onto PFV RNase H. Ribbon diagram of mutant RNase H-(D599N-H724N). The regions highlighted in orange exhibited significant signal changes upon substrate addition. (A) Based on the structure of the human RNase H/substrate complex (PDB: 2QK9) a structural alignment of the PFV and human RNase H was performed to model the RNA/DNA substrate of the human RNase H onto PFV RNase H (Rmsd: 2.35 Å). The RNA strand is shown in dark green, the DNA strand in light green. (B) Alignment of the complex shown in (A) with the HIV-1 RT/RNA-DNA complex (PDB: 1HYS) harboring the PPT RNA-DNA. The PPT substrate is depicted in dark (RNA) and light purple (DNA). The location of the active site residues of PFV RNase H is indicated by a black circle.

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Retrovirology

ISSN: 1742-4690

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