Figure 7

Colocalization of incoming FV capsids with the MTOC. PgTubulin-DsRed transfected HeLa cells were incubated with PFV Gag-eGFP tagged SFVmac Env viruses (SE, SE iCS). The samples were fixed with 3% PFA after 0, 2 or 6 h post virus binding at 37°C, and confocal fluorescence images were collected. Representative images for (A) control cells, (B) pgTubulin-DsRed expressing cells, (C-D) pgTubulin-DsRed transfected and (C) SFVmac Env wt or (D) SFVmac Env iCS incubated cells at 0, 2, 6 h post virus binding are shown. Scale bar: 5 μm