Figure 1

Representative electron micrographs showing the involvement of endocytic pathways in PFV uptake. HeLa cells were incubated with untagged, wildtype PFV particles at ~10°C. After 30 minutes incubation, the cells were (A-B) either fixed (t0) or (C-F) warmed to 37°C for an additional 10 minutes (t1) or 30 minutes (t2) prior to fixation. Scale bars: 100 nm