CTGC motifs within the HIV core promoter specify Tat-responsive pre-initiation complexes

Retrovirology

Table 1 Oligonucleotide sequences for EMSA and pHIV-RL constructs

Name	Sequence
HIV wt	CTAGATGCTGCATATAAGCAGCTGCTTTTTGCATG
TATAKO	CTAGATGCTGAGAGCTCGCAGCTGCTTTTTGCATG
USF-1+	CTAGATGCTGCATATAAGCACGTGCTTTTTGCATG
USF-KO	CTAGATGCTGCATATAAGCGGCCGCTTTTTGCATG
AdMLP	CTAGAGGGGCTATAAAAGGGGGTGGGGGCGGCATG
HSP70	CTAGACGACTTATAAAAGCCCAGGGGCAAGGCATG
CTGC-5’	CTAGATGATCCATATAAGCAGCTGCTTTTTGCATG
CTGC-3’	CTAGATGCTGCATATAAGGATATCCTTTTTGCATG
CTGC-5’3’	CTAGATGATCCATATAAGGATATCCTTTTTGCATG

Primers were designed to create a double-stranded template for radioactive labelling for use in EMSA or for direct cloning into XbaI and SphI sites. For brevity only the positive strand oligos are shown. Bold face type indicates nucleotides mutated from the wild type sequence.

ISSN: 1742-4690