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Figure 2 | Retrovirology

Figure 2

From: Acquisition of CD4-Dependence by CD4-Independent SIV Passaged in Human Peripheral Blood Mononuclear Cells

Figure 2

Progressive changes in CD4 and CKR usage by SIV serially passaged in hPBMCs. A. Day 7 supernatant p27 concentrations of hPBMC cultures, transiently treated with Leu3a anti-CD4 mAb during infection, were normalized to simultaneously mock-treated control cultures (100%, dashed line ------). Initial 4- and 3-fold greater replication in Leu3a anti-CD4 mAb treated cultures of, respectively, SIV mac239 CEMx174 (abbreviated SIV mac239) and SIV 17E-Fr was followed by progressively increasing CD4 dependence during serial passage in unrelated human donor PBMCs. B. By the 15th passage, when both SIV strains had become almost completely CD4 dependent (see A), CKR usage in both strains had switched from roughly equal CXCR4 and CCR5 to nearly exclusive CXCR4 tropism, demonstrated by relative reduction of virus growth in cells pretreated with blocking mAbs against CXCR4 vs. CCR5.

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