Moloney murine leukemia virus glyco-gag facilitates xenotropic murine leukemia virus-related virus replication through human APOBEC3-independent mechanisms

Table 5 Expression of hA3B in 293 T cells infected with XMRV/MX did not affect viral infectivity

Donor cells	Trial 1	Trial 2	Trial 3	Trial 4	Ave ± Std
Donor cells	ICs/ml^*	ICs/ml	ICs/ml	ICs/ml	ICs/ml	Relative infectivity^**
293T/XMRV/pcDNA3	7.0 X 10⁴	l.3 X l0⁵	l.5 X 10⁵	1.5 X 10⁵	l.2 ± 0.37 X l0⁵	1 ± 0
293T/XMRV/hA3B	8.5 X 10⁴	l.0 X l0⁵	l.4 X l0⁵	l.2 X l0⁵	1.1 ± 0.23 X l0⁵	0.931 ± 0.185
293T/MX/pcDNA3	8.4 X 10⁵	1.4 X 10⁶	3.2 X 10⁶	3.3 X 10⁶	2.2 ± 1.2 X 10⁶	16.7 ± 5.91
293T/MX/hA3B	1.0 X 10⁶	l.9 X 10⁶	4.1 X 10⁶	3.3 X 10⁶	2.6 ± 1.4 X 10⁶	19.8 ± 6.22

The infected 293 T cells were transfected with the plasmid encoding HA-tagged hA3B plasmids (293 T/XMRV/hA3B and 239 T/MX/hA3B) or control plasmids (293 T/XMRV/pcDNA3 and 293 T/MX/pcDNA3). HeLa cells were infected with XMRV released from the viral producing cells described above. *The infectious units (IU) were measured from the number of infectious centers that was normalized by the amount of Capsid in viruses and IU per ml was shown. **The relative infectivity to the HeLa cells infected with the virus released from 293 T/XMRV/pcDNA cells was shown.

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