|
Donor cells
|
Trial 1
|
Trial 2
|
Trial 3
|
Trial 4
|
Ave ± Std
|
|---|
|
ICs/ml*
|
ICs/ml
|
ICs/ml
|
ICs/ml
|
ICs/ml
|
Relative infectivity**
|
|---|
|
293T/XMRV/pcDNA3
|
7.0 X 104
|
l.3 X l05
|
l.5 X 105
|
1.5 X 105
|
l.2 ± 0.37 X l05
|
1 ± 0
|
|
293T/XMRV/hA3B
|
8.5 X 104
|
l.0 X l05
|
l.4 X l05
|
l.2 X l05
|
1.1 ± 0.23 X l05
|
0.931 ± 0.185
|
|
293T/MX/pcDNA3
|
8.4 X 105
|
1.4 X 106
|
3.2 X 106
|
3.3 X 106
|
2.2 ± 1.2 X 106
|
16.7 ± 5.91
|
|
293T/MX/hA3B
|
1.0 X 106
|
l.9 X 106
|
4.1 X 106
|
3.3 X 106
|
2.6 ± 1.4 X 106
|
19.8 ± 6.22
|
- The infected 293 T cells were transfected with the plasmid encoding HA-tagged hA3B plasmids (293 T/XMRV/hA3B and 239 T/MX/hA3B) or control plasmids (293 T/XMRV/pcDNA3 and 293 T/MX/pcDNA3). HeLa cells were infected with XMRV released from the viral producing cells described above. *The infectious units (IU) were measured from the number of infectious centers that was normalized by the amount of Capsid in viruses and IU per ml was shown. **The relative infectivity to the HeLa cells infected with the virus released from 293 T/XMRV/pcDNA cells was shown.
