Figure 2

(A) Viperin expression in HIV-1 primary target cells was determined by Western blot analysis. CD4+ T cells and monocytes isolated from two donor-derived peripheral blood mononuclear cells were treated with or without interferon β1b induction (500 IU/ml) for twenty hours. (B) Western blot analysis of endogenous Viperin expression in primary CD4+ T cells and U937 cells treated with interferon β1b (500 IU/ml) was compared to the transient expression of Viperin (3-fold serial dilutions: 0, 0.01, 0.03, 0.1, 0.3, 1.0 μg) transfected in 293T cells.