Figure 4

Depletion of endogenous MOV10 significantly enhances the retrotransposition of endogenous retroelements. (A) Silencing of endogenous MOV10 significantly enhances the retrotransposition of LINE-1, Alu and IAP. As described in Figure 2A, HeLa non-silencing control or MOV10 KD stable cell lines were transfected with retroelement expression plasmids and retrotransposition was quantified. (LINE-1 **p ≤ 0.0056, Alu ***p ≤ 0.0005, IAP **p ≤ 0.0096). (B) MOV10 silencing does not effect neo expression or selection directly. As described in Figure 2B, HeLa non-silencing control or MOV10 KD cells were transfected with the pcDNA3.1-neo control vector and colonies were quantified. Results are normalised to the non-silencing control, which is set at 100%. For (A) a single control bar set at 100% is graphed for simplicity. Values are the mean ± SD of 3 independent experiments. The data were analysed with an unpaired one-tailed t test.