Relationships of PBMC microRNA expression, plasma viral load, and CD4+ T-cell count in HIV-1-infected elite suppressors and viremic patients

Table 1 ID numbers and characteristics of 22 PBMC donors

ID	Status	CD4	VL	NS	TLDA	qPCR
						RT	PA
7	Control			+	+	+
11	Control			+	+	+	+
15	Control			+	+	+	+
22	Control			+	+	+	+
23	Control			+		+	+
24	Control			+	+	+	+
26	Control			+		+	+
27	Control			+	+	+	+
4 (ES4)	ES	678	< 50	+	+	+	+
5 (ES24)	ES	1491	< 50	+	+	+	+
10 (ES6)	ES	1139	< 50	+	+	+	+
12 (ES3)	ES	1241	< 50	+	+	+	+
16 (ES8)	ES	593	< 50	+	+	+	+
20 (ES25)	ES	572	< 50	+		+	+
21 (ES18)	ES	1762	< 50	+	+	+	+
1	Viremic	383	55990		+
6	Viremic	326	44440	+	+	+	+
8	Viremic	348	12480	+		+	+
9	Viremic	475	8190	+	+	+	+
13	Viremic	490	155000	+	+	+	+
14	Viremic	213	105660	+	+	+	+
18	Viremic	413	19680	+	+	+	+

Numerical designations are presented along with status (Control, Elite Suppressor, or Viremic), CD4 counts (ES and Viremic), and Viral Load (VL, copies/mL; < 50 = below the limit of clinical assays). Plus signs in the rightmost columns indicate which platforms were used to assay miRNA in each sample: NanoString (NS), TaqMan low-density array (TLDA), and individual quantitative PCR assay (qPCR). The latter was performed with both reverse-transcribed-only (RT) and reverse-transcribed/pre-amplified (PA) material. For ES, two identification codes are given: first, the numbers used in this study to blind samples, and second, the designation used in previous studies using samples from these patients, including [44, 55, 56].

ISSN: 1742-4690