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Figure 2 | Retrovirology

Figure 2

From: Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus

Figure 2

The DD mutant of FeLV-A (Glasgow-1) displays a higher infectious titre. (A) Three mutants of the FeLV-A (Glasgow-1) molecular clone, possessing mutations at amino acids 83 and(or) 91, were transfected into HEK293T cells. While FeLV-A (Glasgow-1) (A) has the phenotype D83:N91, the mutants DD, ND and NN were D83:D91, N83:D91 and N83:N91 respectively. Viral supernatant was harvested 72 hours post-transfection, filtered and ultracentrifuged, separated by SDS-PAGE, immunoblotted and probed with either anti-gp70 (SU) or anti-p27 (CA). (B) FeLV-A (Glasgow-1) (A) or the DD, ND and NN mutants were transfected into 293ā€‰T cells and the supernatant was recovered. Serial dilutions of each supernatant were prepared and plated onto QN10 Sā€‰+ā€‰L- cells. 72 hours post-infection, foci were scored manually, values represent the mean +/āˆ’ SEM of two independent experiments.

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