Figure 3

Quantitative PCR of RT products (cDNA) from HIV-1 BaL-infected macrophages pretreated or not with rottlerin or accel siRNA against PKC-delta. Macrophages (5x10⁵ cells/well) were pretreated by rottlerin (5 μM) for 30 minutes (A) or with accel siRNAs against PKC-delta for 3 days (B) and then infected with HIV-1 BaL (1 ng p24) for 3 h at 37°C and washed. Cells were then lysed at 12 h and 24 h after infection, and DNA was analyzed by PCR. Amplification of the early (R-U5) and late (pol) products of RT was performed by quantitative PCR (qPCR). Three independent experiments with macrophages from three different donors gave similar results.