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Figure 3 | Retrovirology

Figure 3

From: Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation

Figure 3

Exocyst complex components EXOC1, EXOC2, EXOC3, and EXOC4 co-immunoprecipitate with wild-type, but not mutant Nefs, in Jurkat E6-1 cell lysates. Nef-HA immunocomplexes (right) and input lysates (left) from Jurkat E6-1 cells harvested at 48 h post-transduction with pHAGE virions bearing wild-type (5C, Lane 2) or mutant Nefs (5C-7, 5C-AxxA; Lanes 3 and 4) or empty pHAGE-IRES ZsGreen vector (Lane 1) were immunoblotted to detect endogenous EXOC1, EXOC2, EXOC3, EXOC4, Pak2, and HA-tagged Nef. β-tubulin is included as a loading control.

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