Vpx is Critical for SIVmne infection of pigtail macaques

Retrovirology

Table 1 In situ RNA hybridization (ISH) results from tissues of SIVmne infected pigtail macaques

SIVmne virus	Vpx-WT		ΔVpx		Vpx: y-a		Vpx: 102
Macaque No.	27617	27614	27616	27613	27618	27615	28487	28486
Virus Loads
Peak VL (x 10-6 copies/ml)	230	200	3.1	0	0.2	4.4	0.3	0
VL at Necropsy (x 10-6 copies/ml)	93.5	19.9	3.1	0	0.2	1.5	0	0
CD4 counts
day 0	1057	2053	1277	2179	1823	1511	819	1176
day 10	955	808	2284	2615	2302	1802	1139	1212
day 21	1109	2740	1531	2818	1954	1701	460	869
day 42		1280		3547		1189	864
ISH in Tissue
Rectum	+ (LP, GALT)	ND	+	0	0	0	0	0
Colon	+ (LP, GALT)	ND	+ (GALT)	0	0	0	0	0
Cecum	+ (LP, GALT)	ND	+ (GALT)	0	0	0	0	0
Ileoceccal Junction	+ (LP, GALT)	ND	+ (GALT/LP)	0	+ (GALT)	+ (GALT)	0	0
Ileum	+	ND	0	0	+ (GALT)	0	0	0
Jejunum	+ (LP, GALT)	ND	+ (GALT)	0	0	0	0	0
Mesenteric Node	ND	+	+	0	0	0	0	0
Spleen	+	ND	+ (and LN)	0	0	+	0	0
Peripheral Node	+	ND	+	+	0	+	0	0

ISH was scored as +, SIV RNA expressing cells present; 0, SIV RNA expressing cell not present; ND, not done
Abbreviations: GALT, gut associated lymphoid tissue; LN, lymph node; LP, lamina propria;

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