Figure 7
From: Vpx is Critical for SIVmne infection of pigtail macaques
Interaction of SIV Vpx and X-102 with DCAF1. FLAG-tagged wild type and mutant SIV Vpx expression clones were transfected into 293 T cells, which were treated with 5 μM MG-132 at 24 h post-transfection. After 48 hrs post-transfection cell lysates were immunoprecipitated with anti-FLAG antibody, and analyzed on SDS-PAGE and immunoblot with anti-DCAF1 antibody (top panel). Total cell lysates were analyzed by SDS-PAGE and immunoblot with anti-DCAF1 and anti-FLAG (bottom panels).