Figure 2

Effect of CsA treatment of target human T cells on SIV infection. (A) Effect of CsA treatment of producer human CEM-SS or target human LuSIV cells on viral infectivity. SIVagm, SIVmac, and HIV-1 from producer CEM-SS cells in the absence (Producer CEM-SS cell CsA [-]) or presence of CsA (Producer CEM-SS cell CsA [+]) were used to infect CsA-untreated (Target LuSIV cell CsA [-]) or CsA-treated target LuSIV cells (Target LuSIV cell CsA [+]). Luciferase activity in target LuSIV cells was measured 24 hr after infection. Relative infectivity is shown as the ratio (%) of the luciferase activity to that of viruses produced from CsA-untreated CEM-SS in CsA-untreated LuSIV cells. Mean values and standard deviations in four independent experiments are shown. (B) Effect of CsA treatment of producer or target human cells on viral replication. SIVagm, SIVmac, and HIV-1 from producer CEM-SS cells in the absence (Producer cell CsA [-]) or presence of CsA (Producer cell CsA [+]) were used to infect CsA-untreated (Target cell CsA [-]) or CsA-treated target CEM-SS cells (Target cell CsA [+]). Heat-inactivated virus was used as an infection control. Relative viral cDNA levels are shown as the ratio (%) of the viral cDNA levels to that of virus produced from CsA-untreated CEM-SS in CsA-untreated CEM-SS cells. Mean values and standard deviations in six independent experiments are shown.