Figure 2
Evolution of NC/p1 mutants in absence of PI pressure and its impact on RC and PI resistance. (A) Representation of the evolutionary pathways observed during in vitro evolution experiments with HXB2436E+437T. Nucleotide changes and amino acid changes (bold) as compared to the wild-type virus HXB2 are indicated. (B) Viral replication curves of the different NC/p1 cleavage site mutants: HXB2437T, HXB2435R+436E+437T and HXB2436E+437T+438R as observed during in vitro evolution experiments of HXB2436E+437T and compared to wild-type HXB2. Error bars indicate the standard error of the mean. (C) Representation of the fold increases in phenotypic drug resistance of the different NC/p1 cleavage site mutants: HXB2437T, HXB2435R+436E+437T and HXB2436E+437T+438R as observed during in vitro evolution experiments of HXB2436E+437T and compared to wild-type HXB2. Drug susceptibility to the PI RO033-4649, lopinavir (LPV), and tipranavir (TPV) was determined in the multiple cycle MTT assay.