Figure 2
From: Tailored enrichment strategy detects low abundant small noncoding RNAs in HIV-1 infected cells
Efficiency of hybridization capture to enrich HIV-1 encoded sncRNAs. Comparison of published data using the currents standard protocols (left) with our novel selection strategy (right). Standard protocols with no selection led to a yield of 0.1-1.0% HIV-1 sncRNAs. Numbers above bars indicate the respective literature references [8, 11, 13]. Using our novel method, application of one round of hybridization capture yielded 8.9 ± 5.7% (mean ± SD) HIV-1 sncRNAs (libraries A and B). Performing two consecutive rounds of selection (libraries C-J) optimized the yield to 78.3 ± 7.6% (mean ± SD) HIV-1 sncRNAs.