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Figure 7 | Retrovirology

Figure 7

From: The endogenous retrovirus ENS-1 provides active binding sites for transcription factors in embryonic stem cells that specify extra embryonic tissue

Figure 7

Epigenetic regulations silence the Ens-1 LTR in differentiated cells. cES induced to differentiate 48 h with retinoic acid (Diff) were further treated with TSA (Trichostatin A, 10 nM), VPA (Valproic acid, 100 μM) or 5aza (5-aza-cytidine, 100 μM) in differentiation medium without retinoic acid for 12 h. (A) Expression levels of Ens-1, PouV and Nanog transcripts measured by real time PCR. (B) Expression levels of the Gata and Ets1 transcripts that are repressed during differentiation. Results from three experiments +/- s.d. are represented as percentages of the value obtained in undifferentiated cells (Not Diff). T test: *p < 0.05, **p > 0.05 relative to the values obtained in untreated differentiated cells.

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Retrovirology

ISSN: 1742-4690

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