AnkGAG1D4-mediated inhibitory effect on HIV-1 replication. (A), CAp24 titration. SupT1/Myr+AnkGAG1D4 and SupT1/Myr0AnkGAG1D4 stably expressed the N-myristoylation and non-N-myristoylation versions of the H6MA-CA-binding ankiryn AnkGAG1D4, respectively. SupT1/Myr+AnkA32D3 and SupT1/Myr0AnkA32D3 expressed the N-myristoylation and non-N-myristoylation versions of the αRep-A3-binding ankyrin AnkA32D3, respectively. Cells were infected with HIV-1 NL4-3 at MOI 10, and cell culture supernatants collected at time intervals (5, 7, 9, and 11 days pi) and virus progeny titers determined by CAp24 assays, using ELISA. Results shown are mean (m) from triplicate experiments ± SEM. (B), Viral load. The copy numbers of viral genome were determined in the culture supernatants collected on day-11, using Cobas Amplicor tests. Data presented are from triplicate experiments (m ± SEM). The average values were the following: Control SupT1 cells = 2, 470 × 107 copies/mL; SupT1/Myr+AnkGAG1D4 = 4 × 107; SupT1/Myr0AnkGAG1D4 = 15 × 107 ; SupT1/Myr+AnkA32D3 = 140 × 107 ; SupT1/Myr0AnkA32D3 = 245 × 107.