Figure 11

Ank^GAG1D4-mediated inhibitory effect on HIV-1 replication. (A), CAp24 titration. SupT1/Myr+Ank^GAG1D4 and SupT1/Myr0Ank^GAG1D4 stably expressed the N-myristoylation and non-N-myristoylation versions of the H6MA-CA-binding ankiryn Ank^GAG1D4, respectively. SupT1/Myr+Ank^A32D3 and SupT1/Myr0Ank^A32D3 expressed the N-myristoylation and non-N-myristoylation versions of the αRep-A3-binding ankyrin Ank^A32D3, respectively. Cells were infected with HIV-1 _NL4-3 at MOI 10, and cell culture supernatants collected at time intervals (5, 7, 9, and 11 days pi) and virus progeny titers determined by CAp24 assays, using ELISA. Results shown are mean (m) from triplicate experiments ± SEM. (B), Viral load. The copy numbers of viral genome were determined in the culture supernatants collected on day-11, using Cobas Amplicor tests. Data presented are from triplicate experiments (m ± SEM). The average values were the following: Control SupT1 cells = 2, 470 × 10⁷ copies/mL; SupT1/Myr+Ank^GAG1D4 = 4 × 10⁷; SupT1/Myr0Ank^GAG1D4 = 15 × 10⁷ ; SupT1/Myr+Ank^A32D3 = 140 × 10⁷ ; SupT1/Myr0Ank^A32D3 = 245 × 10⁷.