Figure 4
From: Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR
Loss of PAAF1 or Spt6 leads to histone depletion and recruitment of RNAPII at HIV-1 sequences. (A) HeLa-LTR-luc transfected with siRNA against Spt6, PAAF1 or a control were analyzed by IB using the indicated antibodies. (B) Cells in A were analyzed by ChIP using the indicated antibodies on the HIV promoter (prom) and luciferase region (coding) as indicated. The value for the Scr sample in the promoter region was set to 1. (C) HeLa-LTR-luc transfected with Scr or PAAF1 siRNA, followed by treatment with MG132 as indicated, were analyzed by IB and ChIP using the indicated antibodies. Sequences within the HIV-1 promoter and luciferase regions were amplified by Q-PCR. Values obtained for siScr samples in the promoter region were set to 1. Graphs show mean +/- SD (n = 3).