Figure 12

(A) Chromosome projection mandala depicting the association between NANOG and expressed LINEs in human ES cells. Only one LINE (large blue dot on chromosome 13) was expressed to high level in these cells. (B) The genomic region around the LINE on chromosome 13 is shown with the UCSC Genome Browser, where the linear chromosome is mapped on the horizontal axis. The position of the adjacent HERV-H and HERV-L proviruses is shown. Four biological replicates confirm HERV-H and LINE expression in human ES cells while no expression was detected in K562 cells (only one of the 4 replicates is shown). The direction of transcription was determined by strand-specific sequencing [33]. NANOG bound to both LTRs (represented with black squares along the LTR row) in the adjacent HERV-H. The adjacent HERV-L was not transcribed.