- Meeting abstract
- Open Access
Evidence of STLV 2 and STLV 3 infections in wild living bonobos (P. paniscus ) from the Democratic Republic of Congo
© Ahuka-Mundeke et al; licensee BioMed Central Ltd. 2011
- Published: 6 June 2011
- Infectious Disease
- Phylogenetic Analysis
- Lower Sensitivity
- Fecal Sample
- Additional Analysis
Among the four types of HTLV (1,to 4), only three have their simian counterparts (STLV-1, 2 and 3). STLV-1 and 3 have been found in a large number of captive and wild-living monkeys and great apes from Africa and Asia. STLV-2 was reported only in a limited number of captive bonobos (P. paniscus) from Democratic Republic of Congo (DRC), but so far never documented in wild-living populations.
Between March and July 2010, fecal samples from wild-living bonobos were collected at Malebo forest in the Bandundu province, western DRC. We confirmed the species by mitochondrial (mt) DNA analyses and we screened all samples for STLV infection using a generic PCR allowing amplification of partial fragment in tax (220bp) as well as targeting different fragments in the LTR.
We collected a total of 268 bonobos fecal samples. Overall, 3 (1.1%) samples amplified the tax fragment. Among these, one (Pp5538) was identified as STLV-2 and two (Pp5489 and Pp5560) as STLV-3 by phylogenetic analysis. Additional analysis on the LTR fragment showed that the new STLV-2 from Pp5538 clustered with STLV-2 strains previously described in captive bonobos. All PCR attempts to amplify the LTR fragment of Pp5489 and 5560 samples were unsuccessfull.
Our study shows that faecal samples can be used to screen for STLV infection in endangered apes, although most likely with lower sensitivities. We confirmed STLV-2 infection in wild-living bonobos and showed that bonobos are also infected with STLV-3.
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.