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A FAS -670A/G single nucleotide polymorphism may be associated with HTLV-1 infection and clinical evolution to TSP/HAM

  • Antonio C R Vallinoto1Email author,
  • Bárbara B Santana1,
  • Ethienne Lobato dos Santos1,
  • Rafaela Resplande do Espírito Santo1,
  • Renata Bezerra Hermes1,
  • Rita C Medeiros Sousa2,
  • Izaura M V Cayres Vallinoto1,
  • Luiz F A Machado1,
  • Marluísa O G Ishak1 and
  • Ricardo Ishak1
Retrovirology20118(Suppl 1):A63

https://doi.org/10.1186/1742-4690-8-S1-A63

Published: 6 June 2011

Keywords

Polymerase Chain ReactionSingle Nucleotide PolymorphismRestriction EndonucleaseGenotype FrequencyClinical Evolution

FAS and FASL genes are closely linked to the mechanism of the immune system and several polymorphisms in these genes have been associated with susceptibility to diseases. This study investigated the polymorphisms at positions -670 in the FAS gene, and -169 (IVS3nt169) and -124 (IVS2nt-124) in the FASL gene, among HTLV-1/2 infected subjects. Blood samples from 66 HTLV infected subjects and 192 seronegative individuals were collected, and polymorphisms were analyzed using a polymerase chain reaction (PCR) followed by RFLP analysis using restriction endonucleases. The products were visualized after electrophoresis in 4% agarose gel. The genotype frequencies of the FAS -670 polymorphism showed a higher prevalence of genotype -670GG among HTLV-1 infected subjects (46.97%) as compared to the control group (30.73%; p=0.0162). The comparative analysis between symptomatic and asymptomatic HTLV infected subjects showed a higher prevalence (p=0.0007) of the genotype -670GG among patients with TSP/HAM (50%) as compared to asymptomatic subjects (45%). TCD4+ and TCD8+ lymphocyte counts from HTLV infected and seronegative subjects were compared but no significant association was observed. The proviral load values according to the status of symptomatic and asymptomatic infection carrying different genotypes were compared but showed no statistical significance. The present results suggest that FAS -670 polymorphism seems to be associated with susceptibility to HTLV-1 and may increase the chance to develop TSP/HAM among HTLV-1 infected persons.

Authors’ Affiliations

(1)
Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Brazil
(2)
Hospital Universitário João de Barros Barreto, Belém, Brazil

Copyright

© Vallinoto et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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