Volume 8 Supplement 1

15th International Conference on Human Retroviruses: HTLV and Related Viruses

Open Access

Immunohistochemical and virological features of HTLV-1-associated myosites: a study of 13 patients from West Indies and Africa

  • Marion Desdouits1,
  • Olivier Cassar1,
  • Thierry Maisonobe2,
  • Alexandra Desrames1,
  • Achille Aouba3,
  • Olivier Hermine3,
  • Jacqueline Mikol4,
  • Marc Polivka4,
  • Isabelle Penisson-Besnier5,
  • Pascale Marcorelles6,
  • Fabien Zagnoli7,
  • Thomas Papo8,
  • Arnaud Lacour9,
  • Zahir Amoura2,
  • Julien Haroche2,
  • Patrick Chérin2,
  • Antonio Texeira2,
  • Anne-Sophie Morin10,
  • Franck Mortreux11,
  • Eric Wattel11,
  • Michel Huerre12,
  • Marie-Christine Cumont12,
  • Huot Khun12,
  • Sylviane Bassot1,
  • Sandra Martin-Latil1,
  • Graham Taylor13,
  • Antoine Gessain1,
  • Simona Ozden1 and
  • Pierre-Emmanuel Ceccaldi1, 14Email author
Retrovirology20118(Suppl 1):A41

https://doi.org/10.1186/1742-4690-8-S1-A41

Published: 6 June 2011

Background

HTLV-1 is associated with the onset of various inflammatory diseases such as HTLV-1 Associated Myelopathy / Tropical Spastic Paraparesis, uveitis, infective dermatitis or inflammatory myopathies. Here, we aimed to get new insights into the pathogenesis of HTLV-1 associated inflammatory myopathies (HAIM) by studying muscle biopsy specimens and blood samples from 13 HAIM patients.

Results

Mean age of patients was 52.2 years. 7 patients suffered from polymyositis (PM), and 6, from inclusion body myositis (IBM). Histopathological changes were mild to moderate in most patients. Tumor necrosis factor (TNF)-alpha and myeloid dendritic cells were detected in several patients’ biopsies, and Human Leukocyte Antigen (HLA)-ABC, HLA-DR, and matrix metalloproteinase (MMP-2, -9), in most of them. Perforin was frequently detected but there were no apoptotic myonuclei. By means of in situ hybridization, we detected rare HTLV-1 infected infiltrating cells in the muscle tissue of 4 patients. The virus belonged to the cosmopolitan A subtype, transcontinental subgroup. Plasma anti-HTLV-1 antibodies titers were high, but the proviral load was not elevated when compared to asymptomatic HTLV-1 carriers. Myositis-associated autoantibodies were found in patients with HAIM as well as in HTLV-1 infected controls without HAIM, whereas IFN-gamma plasma levels were elevated in HAIM patients.

Conclusions

We describe 13 cases of HTLV-1 associated myositis, which show the classical anatomopathologic features of idiopathic myositis, with moderate muscle inflammation and atrophy. Proviral load was not elevated, but anti-HTLV-1 antiboides titer and IFN-gamma plasma levels were raised.

Authors’ Affiliations

(1)
Epidemiology and Physiopathology of Oncogenic Viruses Unit, CNRS 3015, Pasteur Institute
(2)
Pitié Salpêtrière Hospital
(3)
Necker Hospital
(4)
Lariboisière Hospital
(5)
University Hospital Center
(6)
University Hospital Center
(7)
Clermont Tonnerre Army Hospital
(8)
Bichat Hospital
(9)
University Hospital Center
(10)
Beaujon Hospital
(11)
Oncology and Biotherapies Unit, Lyon University
(12)
Histotechnolgy and Histopathology Unit, Pasteur Institute
(13)
Faculty of Medicine, Imperial College
(14)
Paris 7 University

Copyright

© Desdouits et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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