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Proviral load does not discriminate patients with Human T-Cell Leukemia/Lymphoma (ATLL) from HTLV-1 carriers with a history of Strongyloidiasis
Retrovirology volume 8, Article number: A253 (2011)
Studies evidences that high proviral loads (PVL) are crucial for the leukemogenesis in the development of ATLL. PVL seems to be stable for years in each individual, but varies substantially among subjects. Previous reports suggest that co-infection of HTLV-1 carriers with S. stercoralis may shorten ATLL onset, thus generating a hypothetical “intermediate state”. We explored this hypothesis by comparing the PVL of ATLL patients and HTLV-1/Strongyloides co-infected individuals.
We determined PVL in three groups: (1) HTLV-1 asymptomatic carriers without a history of Strongyloidiasis [AC] (n=8); (2) virus carriers with previous episodes of Strongyloidiasis [SS] (n=8); and (3) patients with ATLL [AT] (n=8). History of Strongyloidiasis was defined by the patient’s report and a positive result with a ELISA. Peripheral blood mononuclear cells (PBMC) were isolated to measure PVL by Sybr-Green quantitative PCR. PVL was expressed as HTLV-1 tax copy number/104 PBMC. Non-parametric tests were used for the statistical analysis.
The PVL in AT was significantly higher than that in AC [7375±4492SD vs.1698±106SD][p=0.01]. PVL was also higher in SS than in AC [4746±1712SD vs.1698±106SD][p=0.002]. Interestingly, no differences were found between AT and SS [7375±4492SD vs.4746± 1712SD][p=0.16].
Our results support the hypothesis that HTLV-1/S. stercoralis co-infected subjects are at higher risk of developing ATLL as they seem to share with ATLL patients impaired immunological mechanisms to control virus propagation. Further studies are needed to fully characterize the co-infected condition.
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López, G., González, E., Clark, D. et al. Proviral load does not discriminate patients with Human T-Cell Leukemia/Lymphoma (ATLL) from HTLV-1 carriers with a history of Strongyloidiasis. Retrovirology 8, A253 (2011). https://doi.org/10.1186/1742-4690-8-S1-A253
- Peripheral Blood Mononuclear Cell
- Virus Propagation
- Intermediate State
- Asymptomatic Carrier
- Immunological Mechanism