Skip to main content

Advertisement

Successful development of novel monoclonal antibodies against HTLV-1 bZIP factor and their applications in studying the pathogenesis of HAM/TSP

Article metrics

  • 822 Accesses

Background

A recent report has indicated that transgenic expression of HTLV-1 bZIP factor (HBZ) in CD4+ T cells induced T-cell lymphomas and systemic inflammation in mice. However, there is limited information on in vivo expression of HBZ in HTLV-1 infected individuals especially HAM/TSP.

Methods

We have generated rat and human monoclonal antibodies against HBZ by hybridoma methodology and a phage display system, and developed the ELISA system for the detection of HBZ protein and anti-HBZ antibodies. Using these reagents, we analyzed the expression of HBZ protein in plasma and peripheral blood mononuclear cells (PBMCs) of HTLV-1 infected individuals as well as HTLV-1 infected cell lines. Then the results were compared with the real time PCR data.

Results

Although we successfully detected HBZ protein in an enforced overexpressed cells and HTLV-1 infected cell lines by flow cytometry, ELISA, immunofluorescence and immunoblotting, we could not detect HBZ protein or anti-HBZ antibodies in HAM/TSP patients and asymptomatic carrires (ACs) by our system. In contrast, HBZ mRNA was significantly elevated in HAM/TSP patients than ACs. Especially, rapidly progressive HAM/TSP patients showed extremely high expression of HBZ mRNA but not tax mRNA in their PBMCs.

Conclusions

These findings suggest that although expression of HBZ protein was suppressed in vivo in HAM/TSP patients and ACs, high expression of HBZ mRNA is closely associated with the pathogenesis of HAM/TSP.

Author information

Correspondence to Mineki Saito.

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Saito, M., Tanaka, R., Kodama, A. et al. Successful development of novel monoclonal antibodies against HTLV-1 bZIP factor and their applications in studying the pathogenesis of HAM/TSP. Retrovirology 8, A244 (2011) doi:10.1186/1742-4690-8-S1-A244

Download citation

Keywords

  • Lymphoma
  • High Expression
  • Peripheral Blood Mononuclear Cell
  • Systemic Inflammation
  • Transgenic Expression