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Viral caracterisation of “zoonotic” Foamy viruses
Retrovirology volume 8, Article number: A240 (2011)
Simian Foamy Virus is a widespread retrovirus infecting non-human primates (NHP). It is latent in PBMCs but replicates efficiently in saliva. It can be transmitted to humans mainly by bites, giving rise to a lifelong infection. Little is known about FV replication in humans. Genomic changes and quasi-species variability in human PBMCs and saliva have not been extensively studied yet.
Materials and methods
In South Cameroon, a series of hunters bitten either by an African Green Monkey (AGM), a chimpanzee (cpz) or a gorilla (ggo) were found to be SFV-infected. Viral isolation was performed by co-cultivation of their PBMCs with BHK cells. We also analyzed quasi-species (in a 425pb-Pol fragment) from PBMCs and saliva of 9 SFVggo-infected hunters.
5 viral strains (1 SFVagm, 2 SFVcpz and 2 SFVggo) were isolated and sequenced. They are about 5-15% divergent from the corresponding prototypical sequences. Their divergence is s(ub)pecies-specific and no common genomic feature was found between the “zoonotic” strains. Quasi-species variability ranges from 0,3% in saliva to 0,5% in PBMCs. In only 2/9 cases, FV clones are clustered in two groups: PBMCs versus saliva.
In contrast with previous studies, no deletion or specific mutations have been observed in the 5 “zoonotic” FV, suggesting that FV restriction in humans is not due to genetically impaired viruses. Preliminary data indicate that quasi-species variability in saliva seems not higher than in PBMCs, which might be explained by a low replication in human saliva.
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Rua, R., Betsem, E., Calattini, S. et al. Viral caracterisation of “zoonotic” Foamy viruses. Retrovirology 8 (Suppl 1), A240 (2011). https://doi.org/10.1186/1742-4690-8-S1-A240
- Viral Isolation
- Specific Mutation
- Genomic Feature
- Genomic Change
- African Green Monkey