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  • Meeting abstract
  • Open Access

Prevalence of XMRV in blood donors, HTLV and HIV cohorts

  • 1Email author,
  • 1,
  • 2,
  • 2,
  • 1,
  • 1 and
  • 1
Retrovirology20118 (Suppl 1) :A222

https://doi.org/10.1186/1742-4690-8-S1-A222

  • Published:

Keywords

  • Blood Donor
  • Chronic Fatigue Syndrome
  • Chronic Fatigue Syndrome Patient
  • Reactive Sample
  • Immunodominant Region

Background

PCR-based testing has been widely utilized to assess the prevalence of Xenotropic Murine Leukemia Virus-related Virus (XMRV) in prostate cancer and chronic fatigue syndrome patients. An alternative approach, screening for antibodies elicited by XMRV infection, represents a more desirable option for large-scale epidemiologic studies. In this study, blood donor and retrovirus-infected populations were screened for serologic evidence of XMRV infection.

Methods

Plasma from 1000 US blood donors, 100 HIV-1 infected Cameroonians, 486 HTLV-I infected Japanese, and 156 Japanese HTLV-uninfected controls were screened for antibodies to XMRV gp70 and p15E using recombinant-based chemiluminescence immunoassays (CMIAs). CMIA reactive samples were further evaluated by Western Blot (WB) and real-time RT-PCR for XMRV pol and env sequences.

Results

Of US donors, 0.8% (8/1000) were CMIA reactive: 1 p15E and 3 of 7 gp70 reactive samples WB confirmed yielding a 0.4% seroreactive rate. No HIV-1 infected specimens were reactive. Of the Japanese samples, 1/156 uninfected (0.6%) and 20/486 HTLV-infected samples (4.1%) had detectable p15E antibodies by CMIA and WB. Eight additional HTLV-infected samples were gp70 reactive; 4 of 486 (0.8%) were gp70 reactive in WB. No XMRV pol or env sequences were detected in the seroreactives.

Conclusions

XMRV seroprevalence ranged from 0 - 0.6% in US blood donors, HIV-1 infected and HTLV uninfected subjects. Notably, 4.1% of Japanese HTLV-I infected individuals were p15E reactive. Inspection of sequence homology between HTLV and XMRV revealed a high level of conservation within the immunodominant region of HTLV gp21 suggesting increased seroreactivity is due to cross-reactive antibodies.

Authors’ Affiliations

(1)
Abbott Diagnostics, Infectious Diseases, Abbott Park, North Chicago, Illinois 60064, USA
(2)
Abbott Molecular Inc., Des Plaines, IL 60018, USA

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