Skip to content

Advertisement

  • Meeting abstract
  • Open Access

Development of a model of HTLV-1 oral transmission in the rabbit

  • 1,
  • 1,
  • 1,
  • 1 and
  • 2, 3Email author
Retrovirology20118 (Suppl 1) :A19

https://doi.org/10.1186/1742-4690-8-S1-A19

  • Published:

Keywords

  • Post Exposure
  • Mononuclear Leukocyte
  • Antigen Production
  • Oral Model
  • Infected Rabbit

A primary route of transmission of human T-lymphotropic virus 1 (HTLV-1) is from mother-to-child via breast milk, but knowledge of the early immunologic events in orally acquired HTLV-1 infection is limited. Herein, we characterized normal rabbit gut-associated lymphoid tissues (GALT) and performed studies to develop an oral model of HTLV-1 infection. Mononuclear leukocytes were immunophenotyped from key GALT inductive and effector sites using flow cytometry and immunohistochemistry. Our data indicate that unexposed rabbits GALT have a predominant CD4+ lymphocyte population similar to humans. To establish a HTLV-1 oral model 12 week old female New Zealand White rabbits were orally or intravenously inoculated with CD3+CD4+CD25+ rabbit lymphocyte cell line immortalized with the HTLV-1 molecular clone ACH (R-49 cells) or control Jurkat T-cells orally. The rabbits were monitored for hematologic and virologic parameters prior to serial sacrifice. Collectively, 66 to 100% of HTLV-1 orally exposed rabbits became persistently infected. HTLV-1orally exposed and infected rabbits during early time points (1-4 weeks post exposure) had delayed and often less intense anti-HTLV-1 antibody response, variable leukocytosis, and a delayed p19 matrix antigen production and proviral DNA amounts in peripheral blood leukocytes compared to IV exposed rabbits. Interestingly, by 8 weeks post exposure orally exposed rabbits had established similar systemic spread of the virus compared to IV exposed rabbits. This oral model of HTLV-1 transmission in rabbits creates to opportunity to test the role of the mucosal microenvironment during the early stages of orally-acquired HTLV-1 in gut-associated lymphoid tissue.

Authors’ Affiliations

(1)
Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio 43210, USA
(2)
Department of Veterinary Biosciences, Center for Retrovirus Research, The Ohio State University, Columbus, Ohio 43210, USA
(3)
Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Solove Research Institute, The Ohio State University, Columbus, Ohio 43210, USA

Copyright

Advertisement