Volume 8 Supplement 1

15th International Conference on Human Retroviruses: HTLV and Related Viruses

Open Access

Tax-induced rapid senescence is mediated by both classical and alternative NF-κB pathways

  • Yik-Khuan Ho1,
  • Huijun Zhi1,
  • Dominic DeBiaso1,
  • Hsiu-Ming Shih2 and
  • Chou-Zen Giam1Email author
Retrovirology20118(Suppl 1):A189

https://doi.org/10.1186/1742-4690-8-S1-A189

Published: 6 June 2011

HTLV-1 oncoprotein, Tax, is a potent activator of classical and alternative NF-κB signaling pathways and is thought to promote cell proliferation and transformation via NF-κB activation. We have shown recently that hyper-activation of NF-κB by Tax immediately triggers the cellular senescence checkpoint. Down-regulation of NF-κB activation, by contrast, rescues cells from Tax-induced rapid senescence (Tax-IRS). Here, we demonstrate that the IKKalpha and NEMO subunit of the IKK complex are absolutely essential to Tax-IRS. Repression of IKKalpha or NEMO expression by small hairpin RNAs abrogated Tax-mediated activation of both classical and alternative NF-κB pathways and rendered the knockdown cells resistant to Tax-IRS. IKKalpha deficiency moderately affected Tax-induced I-κB-alpha degradation and NF-κB nuclear localization, but drastically repressed NF-κB transcriptional activation. While IKKbeta and TAK1 knockdown significantly attenuated Tax-induced NF-κB transcriptional activation respectively, they only partially prevented Tax-IRS and did not display significant effect on the alternative pathway. Importantly, NIK knockdown yielded similar effects as IKKalpha and NEMO knockdown. These data suggest that Tax, through its interaction with NEMO, help recruit NIK and TAK1 for IKKalpha and IKKbeta activation respectively. Tax-mediated senescence as driven by the NEMO/NIK/IKKalpha complex involves both classical and alternative NF-κB pathways. Thus, the quality rather than the quantity of NF-κB activity is more important for promoting senescence.

Authors’ Affiliations

(1)
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences
(2)
Institute of Biomedical Sciences, Academia Sinica

Copyright

© Ho et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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