Volume 8 Supplement 1

15th International Conference on Human Retroviruses: HTLV and Related Viruses

Open Access

Influence of INF-gamma gene polymorphism in HTLV-1 proviral load

  • Rodrigo Haddad1, 2Email author,
  • Maurício C Rocha–Junior1, 3,
  • Daiani C Cilião-Alves2,
  • Virgínia M D Wagatsuma1,
  • Oswaldo M Takayanagui2,
  • Eduardo A Donadi2,
  • Dimas T Covas1, 2 and
  • Simone Kashima1, 3
Retrovirology20118(Suppl 1):A119

https://doi.org/10.1186/1742-4690-8-S1-A119

Published: 6 June 2011

Background

Interferon-gamma (INF-γ) is a key cytokine involved in the defense against intracellular pathogens, such as HTLV-1, by coordinating the expression of immunologically relevant genes. Previous studies showed the importance of this cytokine on HTLV-1-related pathogenesis. Thus, we have investigated the possible association between IFN-γ gene single-nucleotide polymorphism linked to high and low producer phenotypes (IFN-γ [+874T(high) → A(low)]) and risk of development of symptoms related with HTLV-1 infection and proviral load.

Methods

The polymorphism +874 T/A of INF-γ was analyzed by PCR-SSP in 93 patients HLTV-1 positive (HAC + HAM), stratified according to the presence (HAM, n = 50) or not (HAC, n = 43) of symptoms, and healthy controls (n = 150). Proviral load of infected patients (HAC and HAM) was determined by real-time PCR.

Results

No significant difference was observed for allelic and genotypic frequencies of the +874T/A polymorphism of INF-γ when correlated with HAC, HAM and healthy controls groups. The median of proviral load was lower in HAC than HAM group (p=0.0131). Also, the p-value is very close to significance (p=0.0523) for +874TT genotype (high producer of INF-γ) and low proviral load, compared to the genotypes +874AA and +874AT.

Conclusion

Despite the lack of significant associations, the low proviral load appears correlated with high producer of INF-γ (+874TT) genotype. Increasing the number of patients may lead to statistical relationship of the +874TT genotype with low proviral load.

Declarations

Acknowledgements

Financial Support FAPESP, CNPq, CTC/FUNDHERP and INCTC.

Authors’ Affiliations

(1)
Regional Blood Center of Ribeirão Preto
(2)
Faculty of Medicine of Ribeirão Preto, University of São Paulo
(3)
Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo

Copyright

© Haddad et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement