Skip to content

Advertisement

  • Meeting abstract
  • Open Access

CD3e expression in HTLV-1-infected individuals is associated with proviral load and Tax expression

  • 1, 2Email author,
  • 1, 2,
  • 1,
  • 1, 2,
  • 1, 3,
  • 1,
  • 1,
  • 1,
  • 3,
  • 1, 3 and
  • 1, 2
Retrovirology20118(Suppl 1):A114

https://doi.org/10.1186/1742-4690-8-S1-A114

Published: 6 June 2011

Keywords

  • Gene Expression Profile
  • Hierarchical Cluster
  • Activation Pathway
  • Hierarchical Cluster Analysis
  • Global Gene Expression

Introduction

CD4+ T cells play a central role in HTLV-1 infection. We investigated the global gene expression profile of circulating CD4+ T cells in distinct clinical status of HTLV-1-infected individuals in regard to Tax expression levels.

Methods

The microarray platform used 12 individual samples divided according to patients’ clinical status and Tax expression as follows: healthy control (CT, n=4), HAC (n=4, 2 high Tax expression and 2 low Tax expression) and HAM/TSP group (n=4, 2 high Tax expression and 2 low Tax expression). Proviral load (PVL) was quantified by qRT-PCR and Tax expression was analyzed by flow cytometry in HAC and HAM/TSP group.

Results

Hierarchical clustering analysis showed that CT and HTLV-infected groups clustered separately. We also observed that HAC and HAM/TSP groups were in separate clusters regardless Tax expression. We identified 449 genes differentially expressed between HAC and HAM/TSP groups and we classified these genes according the biological functions. CD3e was represented in many functions like cellular development, cell signaling, and others. CD3e expression by qRT-PCR was higher (1.3X) in the HAM/TSP than HAC group (p=0.0195). We also validated LCK, VAV and ZAP70 genes, which are downstream molecules of the CD3e activation pathway. These genes were also significantly higher in HAM/TSP group and CD3e, LCK and VAV1 genes were positively correlated with PVL and Tax expression.

Conclusion

The higher PVL and Tax expression the higher activity of CD4+ T cells in the symptomatic group, suggesting that this pathway could have an important role in HAM/TSP development.

Declarations

Acknowledgements

Financial support: FUNDHERP, CTC, INCTC, FAPESP, CNPq and CAPES.

Authors’ Affiliations

(1)
Regional Blood Center of Ribeirão Preto, Ribeirão Preto, Brazil
(2)
Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
(3)
Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

Copyright

© Pinto et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement