Table 4 Oligonucleotides used for NL4.3 GFP E- quantitative PCR (Butler 2001).
| Â | Primer name | Primer sequence |
|---|---|---|
Late RT | MH531 | 5'-GCATACATTATACGAAGTTATGCTGC-3' |
| Â | MH532 | 5'-GCCGTGCGCGCTTCAGCAAGC-3' |
| Â | LRT-P | 5'-(FAM)-CAGTGGCGCCCGAACAGGGA-(TAMRA)-3' |
2-LTR | MH535 | 5'-AACTAGGGAACCCACTGCTTAAG-3' |
| Â | MH536 | 5'-TCCACAGATCAAGGATATCTTGTC-3' |
| Â | MH603 probe | 5'-(FAM)-ACACTACTTGAAGCACTCAAGGCAAGCTTT-(TAMRA)-3' |
Alu PCR | MH535 | 5'-AACTAGGGAACCCACTGCTTAAG-3' |
| Â | SB704 rev | 5'-TGCTGGGATTACAGGCGTGAG-3' |
| Â | MH603 probe | 5'-(FAM)-ACACTACTTGAAGCACTCAAGGCAAGCTTT-(TAMRA)-3' |