Table 2 Oligonucleotides used for cloning in this study.

E45A fwd

5'-CAGCATTATCAGCTGGAGCCACCCC-3'

E45A rev

5'-GGGGTGGCTCCAGCTGATAATGCTG-3'

T54A fwd

5'-CCACAAGATTTAAACGCCATGCTAAACACAGTGG-3'

T54A rev

5'-CCACTGTGTTTAGCATGGCGTTTAAATCTTGTGG-3'

T54A/N57A fwd

5'- CCACAAGATTTAAACGCCATGCTAGCCACAGTGGGGGGAC-3'

T54A/N57A rev

5'-GTCCCCCCACTGTGGCTAGCATGGCGTTTAAATCTTGTGG-3'

N57A fwd

5'-CACCATGCTAGCCACAGTGGGGGGAC-3'

N57A rev

5'-GTCCCCCCACTGTGGCTAGCATGGTG-3'

Q63A/Q67A fwd

5'-GTGGGGGGACATGCAGCAGCCATGGCAATGTTAAAAGAGAC-3'

Q63A/Q67A rev

5'-GTCTCTTTTAACATTGCCATGGCTGCTGCATGTCCCCCCAC-3'

K70R fwd

5'-GCAGCCATGCAAATGTTAAGAGAGACCATCAATGAGGAAG-3'

K70R rev

5'-CTTCCTCATTGATGGTCTCTCTTAACATTTGCATGGCTGC-3'

N74D fwd

5'-GAGACCATCGATGAGGAAGCTGCAGAATGG-3'

N74D rev

5'-CCATTCTGCAGCTTCCTCATCGATGGTCTC-3'

G89V fwd

5'-CCAGTGCATGCAGTGCCTATTGCACCAGGCCAG-3'

G89V rev

5'-CTGGCCTGGTGCAATAGGCACTGCATGCACTGG-3'

G89V/A92E fwd

5'-CCAGTGCATGCAGTGCCAATTGAGCCAGGCCAGATGAG-3'

G89V/A92E rev

5'-CTCATCTGGCCTGGCTCAATTGGCACTGCATGCACTGG-3'

P90A fwd

5'-CCAGTGCATGCAGGTGCCATTGCACCAGGCCAGATG-3'

P90A rev

5'-CATCTGGCCTGGTGCAATGGCACCTGCATGCACTGG-3'

P90A/A92E fwd

5'-CCAGTGCATGCAGGCGCAATTGAGCCAGGCCAGATG-3'

P90A/A92E rev

5'-CATCTGGCCTGGCTCAATTGCGCCTGCATGCACTGG-3'

A92E fwd

5'-GTGCATGCAGGGCCAATTGAGCCAGGCCAGATG-3'

A92E rev

5'-CATCTGGCCTGGCTCAATTGGCCCTGCATGCAC-3'

G94D fwd

5'-GTGCATGCAGGGCCCATTGCACCAGACCAGATGAGAGAACC-3'

G94D rev

5'-GGTTCTCTCATCTGGTCTGGTGCAATGGGCCCTGCATGCAC-3'

A105T fwd

5'-GGAAGTGACATAACAGGAACTACTAGTACC-3'

A105T rev

5'-GGTACTAGTAGTTCCTGCTATGTCACTTCC-3'

K131R fwd

5'-CCAGTAGGAGAAATCTATAGAAGATGGATAATCCTGG-3'

K131R rev

5'-CCAGGATTATCCATCTTCTATAGATTTCTCCTACTGG-3'

R132K fwd

5'-GGAGAAATCTATAAAAAATGGATAATCCTGGG-3'

R132K rev

5'-CCCAGGATTATCCATTTTTTATAGATTTCTCC-3'

K140R fwd

5'-GGATAATCCTGGGATTAAATAGAATAGTAAGAATGTATAGC-3'

K140R rev

5'-GCTATACATTCTTACTATTCTATTTAATCCCAGGATTATCC-3'

K158R fwd

5'-GACATAAGACAAGGACCAAGGGAACCCTTTAGAGACTATG-3'

K158R rev

5'-CATAGTCTCTAAAGGGTTCCCTTGGTCCTTGTCTTATGTC-3'

K170R fwd

5'-CTATGTAGACCGATTCTATAGAACTCTAAGAGCCGAGC-3'

K170R rev

5'-GCTCGGCTCTTAGAGTTCTATAGAATCGGTCTACATAG-3'

K182R fwd

5'-CAAGCTTCACAAGAGGTAAGAAATTGGATGACAGAAACC-3'

K182R rev

5'-GGTTTCTGTCATCCAATTTCTTACCTCTTGTGAAGCTTG-3'

NotI fwd

5'-CCTTGGCTTCTTATGCGACGG-3'

SpeI rev

5'-GTCCAGAATGCTGGTAGGGC-3'

SpeI fwd

5'-GCTGCAGAATGGGATAGAGTGC-3'

ApaI rev

5'-GTGGGAAGGCCAGATCTTCC-3'

D185K/D186K fwd

5'-GACATAGTCATCTATCAATACATGAAGCTTTTGTATGTAGGATCTGACT TAG-3'

D185K/D186K rev

5'-CTAAGTCAGATCCTACATACAAAAGCTTCATGTATTGATAGATGACTA TGTC-3'

EcoRV fwd

5'-GTTCCCTTAGATAAAGACTTCAGG-3'

AgeI rev

5'-GATATGTCCATTGGCCTTGC-3'

D116A fwd

5'-CAGTAAAAACAGTACATACAGCCAATGGCAGCAATTTCACC-3'

D116A rev

5'-GGTGAAATTGCTGCCATTGGCTGTATGTACTGTTTTTACTG-3'

AgeI fwd

5'-CCAAAGCACTAACAGAAGTAGTACC-3'

EcoRI rev

5'-CGAGTAACGCCTATTCTGCTATG-3'

ntTNPO3 fwd

5'-GTTGGTTTAACTTGGGAGTTTTAGATTCGAATTTTATGGCTAACAATAA ATTACTAGCAC-3'

ntTNPO3 rev

5'-GTGCTAGTAATTTATTGTTAGCCATAAAATTCGAATCTAAAACTCCCAA GTTAAACCAAC-3'

AflII fwd

5'-TGTGTGCTTAAGCTCGAGTGGTGGCATGGAAGGAGCAAAGC-3'

KpnI rev

5-GTAAGCTTTGAAGATGCCATG-3'

CMV fwd

5'- TGTGTGTTAATTAAGCGTTGACATTGATTATTGACTAG-3'

CMV rev

5'- CACACACTCGAGCCCTTGTCATCGTCGTCCTTGTAGTCCATGTTTAAA CGCTAGCCAGCTTG-3'