Figure 7
Effect of TNPO3 KD by transfection of siRNA oligonucleotides on HIV-1 transduction and cDNA synthesis. HeLa cells were transfected with the indicated siRNAs and challenged 72 hrs later with HIV-1NL4-3GFP reporter virus. (A). Cell lysate was probed in western blots with anti-TNPO3 antibody (upper panel) and anti-β-actin antibody (lower panel). (B) HIV-1NL4-3GFP reporter gene expression was checked by flow cytometry 72 hrs after virus challenge. 24 hrs after challenge with HIV-1NL4-3GFP, cell-associated DNA was harvested and processed by qPCR for late RT (C), 2-LTR circles (D) and provirus (E). Azidothymidine (AZT) and Raltegravir (RAL) were used to block de novo reverse transcription and integration, respectively. Data represent one of at least three independent experiments. Error bars represent ± SEM (n = 3).