Figure 6

Effect of TNPO3 on the de novo synthesis and fate of HIV-1 cDNA after acute infection. WT or CA mutant HIV-1 reporter virus were used to challenge the TNPO3 KD and rescue cells, as indicated. 24 hrs later, late RT (A), 2-LTR circles (B) and provirus (C) were assayed by qPCR. Viruses bearing the enzymatic site mutants RT-D185K/D186L or IN-D116A IN were used as controls for de novo reverse transcription and integration, respectively. Data represent one of at least three independent experiments. Error bars represent ± SEM (n = 3).