Table 1 Characterization of proteasome antibodies for immunofluorescence.
From: TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions
Proteasome Antibodies | |||
|---|---|---|---|
19S Base | Catalog Number | Ab Nuclear Localization | Locialization with rhTRIM5a |
RPT1 mouse | BML-PW8825 | INTERMEDIATE | YES |
RPT1 rabbit | BML-PW8165 | INTERMEDIATE | YES |
RPT2 rabbit | BML-PW8305 | NO | NO |
RPT3 mouse | BML-PW8220 | YES | NO |
RPT3 rabbit | BML-PW8175 | INTERMEDIATE | YES |
RPT4 mouse | BML-PW8310 | INTERMEDIATE | NO |
RPT4 rabbit | BML-PW8830 | NO | NO |
RPT5 mouse | BML-PW8770 | YES | YES |
RPT5 rabbit | BML-PW8765 | NO | NO |
RPT6 rabbit | BML-PW8320 | YES | NO |
20S Alpha | |||
2 mouse | BML-PW8105 | YES | YES |
4 mouse | BML-PW8120 | YES | YES |
5 mouse | BML-PW8125 | NO | NO |
6 mouse | BML-PW8100 | YES | YES |
7 mouse | BML-PW8110 | INTERMEDIATE | NO |
Core rabbit | BML-PW8155 | YES | YES |
Alpha 1,2,3,5,6 & 7 mouse | BML-PW8195 | NO | NO |
20S Beta | |||
1i (LMP2-13) rabbit | BML-PW8345 | NO | NO |
1 mouse | BML-PW8140 | NO | NO |
2 mouse | BML-PW8145 | NO | NO |
2i (MECL-1) rabbit | BML-PW8350 | YES | NO |
3 mouse | BML-PW8130 | INTERMEDIATE | NO |
4 rabbit | BML-PW8890 | NO | NO |
5i (LMP7) mouse | BML-PW8845 | NO | NO |
5i (LMP7) rabbit | BML-PW8355 | YES | NO |
5 rabbit | BML-PW8895 | NO | NO |
6 rabbit | BML-PW9000 | NO | NO |
7 mouse | BML-PW8135 | NO | NO |