Figure 2
From: TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions
TRIM5α specifically co-immunoprecipitates with proteasome subunits. (A) Co-immunoprecipitation of PSMC2 with rhTRIM5α. 293T cells were transfected with HA-rhTRIM5α and FLAG-PSMC2 or pcDNA3.1 empty vector and FLAG-PSMC2 and subjected to immunoprecipitation (IP) with an HA antibody followed by Western blot using indicated antibodies. (B) Co-immunoprecipitation of RPN8 with rhTRIM5α. 293T cells were transfected with HA-rhTRIM5α and FLAG-RPN8 or pcDNA3.1 empty vector and FLAG-RPN8 and subjected to immunoprecipitation (IP) with an HA antibody followed by Western blot using indicated antibodies. (C) Co-immunoprecipitation of RPT3 with rhTRIM5α. 293T cells were transfected with HA-rhTRIM5α and FLAG-RPT3 or pcDNA3.1 empty vector and FLAG-RPT3 and subjected to immunoprecipitation (IP) with an HA antibody followed by Western blot using indicated antibodies. (D) Co-immunoprecipitation of RPT6 with rhTRIM5α. 293T cells were transfected with HA-rhTRIM5α and FLAG-RPT6 or pcDNA3.1 empty vector and FLAG-RPT6 and subjected to immunoprecipitation (IP) with an HA antibody followed by Western blot using indicated antibodies. (E) 293T cells were transfected with HA-MLK-3 and subjected to immunoprecipitation with an HA antibody followed by Western Blot. (F) Co-immunoprecipitation of PSMC2 with huTRIM5α. 293T cells were transfected with Myc-rhTRIM5α and FLAG-PSMC2 or pcDNA3.1 empty vector and FLAG-PSMC2 and subjected to immunoprecipitation (IP) with a FLAG antibody followed by Western blot using indicated antibodies.