Figure 1

HIV infection does not alter CD127 expression on thymocytes. A) Unfractionated thymocytes (gate 1) were identified based on the forward scatter/side scatter profiles of live cells. The cells were then gated on either CD3^- (gate 2) or CD3⁺ (gate 3) in a single parameter histogram. The cells in gate 2 and gate 3 were then analysed for CD4 and CD8 expression. The expression of CD127-PE (Beckman Coulter) was measured on the various subsets. Thymocytes were incubated with HIV _cs204 or mock infected and co-cultured with thymic epithelial cells for up to 96 hours. Light grey lines represent isotype control, mock infected (black line) and HIV infected (dark grey line). B)SP4 subset C)SP8 subset. Summary data of CD127 expression on thymocyte measured as the proportion of cells expressing CD127 relative to mock infected cultures on D) unfractionated thymocytes, E) SP4, and F) SP8 thymic subsets.