Figure 1

The ability of Nef to associate with PAK2 is not important for enhancing viral infectivity. A. Key residues in the amino acid sequence of Nef variants used in this study. B. Primary Nefs and mutant Nefs were inserted into a modified NL4-3 provirus via a BamHI site in Env and an artificial ClaI site in the viral LTR. SF2 Nef was inserted via an artificial MluI site at the start of Nef and the ClaI site. C and D. MAGI R5+ cells were incubated with 5,000 ³H cpm of RT activity of each viral variant for 6 h. At 36 h post-infection, the cells were fixed and stained for β-galactosidase activity. Average percent of infected (β-galactosidase+) cells from triplicate infections +/standard errors of the mean (SEM) are shown.