DCAF1 (VPRBP) knockdown does not prevent Vpx rescue of HIV-1 from the antiviral state in MDDCs. MDDCs were transduced with lentiviral knockdown vectors targeting either DCAF1, or a control RNA, in the presence of SIV VLPs. DCAF1 KD and control KD cells were then treated with IFN-β for 24 hrs, and lysates were probed in immunoblots with antibodies against the indicated proteins (A), or cells were challenged with a VSV-G-pseudotyped HIV-1NL4-3 GFP reporter virus (B). (C) DCAF1 KD and control KD MDDCs were treated with LPS for 24 h, and challenged with either VSV-G-pseudotyped HIV-1NL4-3 or SIVMAC-239 GFP reporter viruses. Data represent one of at least three independent experiments. Error bars represent ± SD (n = 3).