Analysis of CD4, CXCR4 and CCR5 expression in MSCs. Analysis of CD4, CXCR4, CCR5 and β-actin mRNA expression by qualitative real time RT-PCR in MSCs (A). A typical gel electrophoresis of qualitative real time RT-PCR is shown. As positive controls, total RNAs extracted from PBMC were employed. The total RNAs extracted from NK-92 cells (for CD4) and E. coli total RNA (for CXCR4 and CCR5) served as negative control. Panel B displays a typical flow cytometry analysis of CCR5, CXCR4 and CD4 staining in MSCs. Unshadowed areas represent MSCs treated with FITC-conjugated specific mAb, whereas the negative control (MSCs stained by FITC-conjugated irrelevant isotype matched mAb) is represented by shadowed areas. Three experiments were performed in duplicate.