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Figure 6 | Retrovirology

Figure 6

From: Mutagenesis of tyrosine and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and infectivity

Figure 6

Incorporation of Env glycoproteins into virions. NL4-3 viruses containing the WT and mutant Env proteins were produced in 293T cells and pelleted through a 25% sucrose cushion. Resuspended viral pellets were then subjected to p24 ELISAs, gp120 ELISAs, and gp41 western blotting. Incorporation of Env into virions is calculated as the ratio of gp120/p24 (A) and gp41/p24 (B) and shown as the percentage of WT. The data represents results from three independent experiments conducted in triplicate. The error bars represent the standard deviation of the means.

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Retrovirology

ISSN: 1742-4690

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