Distinctive receptor binding properties of the surface glycoprotein of a natural Feline Leukemia Virus isolate with unusual disease spectrum

Table 1 Summary of comparative flow cytometric binding assays performed using soluble SU proteins of FeLV-A/61E, 61E/945-VRB or mutant FeLV-A/61E SU proteins substituted of specific amino acids within and surrounding the consensus VRB domain of FeLV-945

Soluble SU Protein^a	Average GMF of Replicate Binding Assays^b	Comparable Binding Phenotype
FeLV-A/61E	12.49	N/A
61E/945-VRB	22.67	N/A
VRB3aa	8.64	FeLV-A/61E
N147S	12.16	FeLV-A/61E
K128N/S130T	11.92	FeLV-A/61E
I156V/K164R	10.09	FeLV-A/61E
K128N/S130T/I186V	22.65	61E/945-VRB
I156V/K164R/I186V	21.69	61E/945-VRB

^aComparative flow cytometric binding assays were performed using FeLV-A/61E, 61E/945-VRB, or mutated SU proteins named according to the individual FeLV-945 amino acid residues substituted into FeLV-A/61E as shown in Figure 7C. Binding assays were performed using equivalent mass amounts of each SU protein and feline 3201 cells.
^bThe average geometric mean fluorescence (GMF) is shown for three (N147S), four (VRB3aa, I156V/K164R, K128N/S130T, I156V/K164R/I186V), or five (K128N/S130T/I186V) replicate binding assays using three or four independently generated and titered batches of SU proteins.

ISSN: 1742-4690