Figure 1

ADAM10 silencing inhibits both CCR5- and CXCR4-tropic HIV-1 replication. (A) Primary macrophages were transfected with different siRNAs 48 h prior to infection with HIV-SF162. Azidothymidine (AZT) treatment was used as a positive control to block infection. Supernatants were collected 7 days after infection and HIV p24 production was measured by ELISA. ADAM10 silencing significantly reduced viral replication in primary human macrophages (**P < 0.01). (B) To determine whether ADAM17 also plays a role in HIV-1 replication, TZM-bl cells were transfected with ADAM17, ADAM10 or ERBB2IP siRNAs 48 h prior to infection with LAV, and supernatant HIV p24 was measured by ELISA 3 days after infection (*P < 0.05).