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Figure 5 | Retrovirology

Figure 5

From: Identification of the protease cleavage sites in a reconstituted Gag polyprotein of an HERV-K(HML-2) element

Figure 5

Cleavage site determination by Nterminal sequencing. (A) Proteins from RP-HPLC fractions known to contain mature Pr74Gag subdomains were blotted on a PVDF membrane and made visible by staining with Ponceau S. Protein bands corresponding to the specific sizes of processed Gag domains were cut out (bands framed with black boxes) and sent for Edman degradation to determine the N-terminal amino acid sequence. (B) Western blot analysis of oricoHERVK113_ GagProPol mutants carrying amino acid changes at the P1 position of the MASP1 site (Y134A, lane 1), the CA-NC site (G532D, lane 2) and the p15- CA site (F282D, lane 3). VLPs with wild type (wt) cleavage sites were run in lane 4.

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